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KMID : 0364819910290040216
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Korean Journal of Microbiology
1991 Volume.29 No. 4 p.216 ~ p.229
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Restriction Map of the R Plasmid pKU10 in Pseudomonas putida
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Chun, Sung-Hee/전성희
Lim, Young-Bock/Sim, Woong-Seop/Lee, Yung-Nok/임영복/심용섭/이영록
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Abstract
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1
In our laboratory a R plasmid pKU10 was isolated from Pseudomonas and its characteristics were investigated. In this study, as a basic work to improve its utility as a cloning vehicle, restriction patterns of pKU10 were analyzed for other various restriction enzymes in addition to restriction endonuckases previously examined. As a result, pKU10 DNA has two cleavage sites for Gal and HpaI, and three sites for AvaI. The restriction map of pKU10 was supplemented with Aval, ClaI, and Hpal. From the result of this experiment, the usefulness of pKU10 as a cloning vector in Pseudomonas will be enhanced by constructions of mini-plasmid or hybrid plasmids.
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